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Category:BiosBinding, entry and intracellular trafficking of FGF2 and FGF2-liposomes in Rat C6 glioma cells.
Fibroblast growth factors (FGFs) play important roles in morphogenesis, angiogenesis, and regeneration. FGF2 (also called basic FGF) stimulates proliferation of many cell types, including glial cells, and its receptor FGFR1 is expressed in the central nervous system. We examined whether FGF2 could cross the blood-brain barrier (BBB), in vitro. Transport across the BBB was measured with radiolabelled FGF2 (14 C-labeled FGF2) and FGF2-liposomes, and its receptors, FGFR1 and FGFR2, were detected by Western blot. FGF2 transport was saturable, and it was transported across the BBB from brain to blood with a Km of 1.24 x 10(-7) M and a Vmax of 1.39 x 10(-9) mol/cm2/h. FGF2-liposomes were taken up efficiently and were transported to the endosomes. FGF2 and FGF2-liposomes were transported into the glioma cell line, C6. Western blot confirmed the expression of FGFR1 and FGFR2 in C6 cells.Effects of poly(ethylene glycol) on the platelet aggregation induced by thrombin.
Effects of various poly(ethylene glycol) (PEG) derivatives on the human platelet aggregation induced by thrombin were studied. Poly(ethylene glycol) 400 (PEG-400), poly(ethylene glycol) 4000 (PEG-4000) and poly(ethylene glycol) 20,000 (PEG-20,000) did not affect platelet aggregation induced by thrombin, but PEG-6000 and PEG-30,000 inhibited aggregation. The effect of PEG-6000 on aggregation was concentration-dependent. In contrast, PEG-30,000 inhibited aggregation even at 0.1 mM. The inhibition was relatively rapid in onset and was reversible. The effects of PEG-6000 and PEG-30,000 on thrombin-induced platelet aggregation were significantly reversed by 2.5 mM of Ca2+ ions. The inhibitory effects of PEG-6000 and PEG-30, be359ba680
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